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SHP2 MAC-KO can affect the expression of PI3K/PLCγ signaling pathway, the expression of inflammatory factors and proteins related to phagocytic activity. ( A ) Protein band diagram of p-SHP2, p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κB, TNFα, IFNγ, IL-1β; ( B ) Relative protein expression of p-SHP2, p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κB, TNFα, IFNγ, IL-1β. **P<0.01.

Journal: Aging (Albany NY)

Article Title: Myeloid-specific knockout of SHP2 regulates PI3K/PLCγ signaling pathway to protect against early myocardial infarction injury

doi: 10.18632/aging.205096

Figure Lengend Snippet: SHP2 MAC-KO can affect the expression of PI3K/PLCγ signaling pathway, the expression of inflammatory factors and proteins related to phagocytic activity. ( A ) Protein band diagram of p-SHP2, p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κB, TNFα, IFNγ, IL-1β; ( B ) Relative protein expression of p-SHP2, p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κB, TNFα, IFNγ, IL-1β. **P<0.01.

Article Snippet: Then the membrane was transferred, and put in the TBST buffer containing 5% skim milk powder, blocked for 2 h. Primary antibodies p-SHP2(Abcam, ab62322, 1:1000), p-PI3K (CellSignaling, 17366, 1:1000), p-MerTK (CellSignaling, 44463,1:1000), p-PLCγ2 (CellSignaling, 3871, 1:1000), p-Myd88 (CellSignaling, 17366, 1:1000), p-NF-κB (CellSignaling, 3303,1:1000), TNFα (CellSignaling, 11948, 1:1000), IFNγ (CellSignaling, 8455, 1:1000), IL-1β (CellSignaling, 12703, 1:1000), and GADPH (CellSignaling, 5174, 1:1000) were added, and were incubated at room temperature for 1 hour, then incubated at 4° C overnight.

Techniques: Expressing, Activity Assay

SHP2 MAC-KO can mediate the PI3K/PLCγ signaling pathway to influence the expression of inflammatory factors and proteins related to phagocytic activity. ( A ) Protein band diagram of p-PI3K, p-MerTK, p-PLC Γ2, p-MyD88, p-NF-κb, TNF α, IFN γ, il-1β; ( B ) Relative protein expression amounts of p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κb, TNF α, IL-1β. **P<0.01, ns P>0.05.

Journal: Aging (Albany NY)

Article Title: Myeloid-specific knockout of SHP2 regulates PI3K/PLCγ signaling pathway to protect against early myocardial infarction injury

doi: 10.18632/aging.205096

Figure Lengend Snippet: SHP2 MAC-KO can mediate the PI3K/PLCγ signaling pathway to influence the expression of inflammatory factors and proteins related to phagocytic activity. ( A ) Protein band diagram of p-PI3K, p-MerTK, p-PLC Γ2, p-MyD88, p-NF-κb, TNF α, IFN γ, il-1β; ( B ) Relative protein expression amounts of p-PI3K, p-MerTK, p-PLCγ2, p-MyD88, p-NF-κb, TNF α, IL-1β. **P<0.01, ns P>0.05.

Article Snippet: Then the membrane was transferred, and put in the TBST buffer containing 5% skim milk powder, blocked for 2 h. Primary antibodies p-SHP2(Abcam, ab62322, 1:1000), p-PI3K (CellSignaling, 17366, 1:1000), p-MerTK (CellSignaling, 44463,1:1000), p-PLCγ2 (CellSignaling, 3871, 1:1000), p-Myd88 (CellSignaling, 17366, 1:1000), p-NF-κB (CellSignaling, 3303,1:1000), TNFα (CellSignaling, 11948, 1:1000), IFNγ (CellSignaling, 8455, 1:1000), IL-1β (CellSignaling, 12703, 1:1000), and GADPH (CellSignaling, 5174, 1:1000) were added, and were incubated at room temperature for 1 hour, then incubated at 4° C overnight.

Techniques: Expressing, Activity Assay